{"code":"C99481","name":"Pyrosequencing","terminology":"ncit","version":"26.09d","conceptStatus":"DEFAULT","leaf":true,"active":true,"synonyms":[{"name":"PYROSEQUENCING","termType":"PT","type":"FULL_SYN","typeCode":"P90","source":"CDISC"},{"name":"Pyrosequencing","termType":"PT","type":"FULL_SYN","typeCode":"P90","source":"NCI"},{"name":"Pyrosequencing","type":"Preferred_Name","typeCode":"P108"}],"definitions":[{"definition":"A DNA sequencing method that uses a \"sequencing by synthesis\" strategy. The strand complementary to a template is synthesized a single base at a time through the sequential addition of individual solutions of each base. Incorporation of a base into the growing strand generates free pyrophosphate that is detected as light, as generated by a luciferase-catalyzed reaction.","code":"P97","type":"DEFINITION","source":"NCI"},{"definition":"A DNA sequencing technique that is based on the detection of released pyrophosphate during DNA synthesis.","code":"P325","type":"ALT_DEFINITION","source":"CDISC"}],"properties":[{"code":"P322","type":"Contributing_Source","value":"CDISC"},{"code":"P106","type":"Semantic_Type","value":"Molecular Biology Research Technique"},{"code":"P207","type":"UMLS_CUI","value":"C2737269"}]}