{"code":"C28408","name":"Quantitative Reverse Transcriptase PCR","terminology":"ncit","version":"26.09d","conceptStatus":"DEFAULT","leaf":true,"active":true,"synonyms":[{"name":"qRT-PCR","termType":"SY","type":"FULL_SYN","typeCode":"P90","source":"CDISC"},{"name":"QUANTITATIVE REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION","termType":"PT","type":"FULL_SYN","typeCode":"P90","source":"CDISC"},{"name":"Real-Time Reverse Transcriptase PCR","termType":"SY","type":"FULL_SYN","typeCode":"P90","source":"CDISC"},{"name":"rRT-PCR","termType":"SY","type":"FULL_SYN","typeCode":"P90","source":"CDISC"},{"name":"Quantitative Reverse Transcriptase PCR","termType":"DN","type":"FULL_SYN","typeCode":"P90","source":"CTRP"},{"name":"qRTPCR","termType":"SY","type":"FULL_SYN","typeCode":"P90","source":"NCI"},{"name":"Quantitative Reverse Transcriptase PCR","termType":"PT","type":"FULL_SYN","typeCode":"P90","source":"NCI"},{"name":"Quantitative RTPCR","termType":"SY","type":"FULL_SYN","typeCode":"P90","source":"NCI"},{"name":"Quantitative Reverse Transcriptase PCR","type":"Display_Name","typeCode":"P107"},{"name":"Quantitative Reverse Transcriptase PCR","type":"Preferred_Name","typeCode":"P108"}],"definitions":[{"definition":"A modified polymerase chain reaction (PCR) technique in which RNA sequences are reverse transcribed into complementary DNA (cDNA) transcripts by the enzyme reverse transcriptase. The cDNA is then amplified by a real time PCR reaction. The level of expression of a target gene is calculated using the crossing thresholds (CTs) or crossing points (CPs) which correspond to the numbers of cycles required for a target gene and the housekeeping, internal control gene, to reach a defined fluorescence intensity.","code":"P325","type":"ALT_DEFINITION","source":"CDISC"},{"definition":"Quantitative PCR: a method to measure very small amounts of RNA transcripts as cDNA targets. There are two strategies currently used, one is called Competitive RTPCR and the other is called Real-Time PCR.","code":"P97","type":"DEFINITION","source":"NCI"}],"properties":[{"code":"P322","type":"Contributing_Source","value":"CDISC"},{"code":"P322","type":"Contributing_Source","value":"CTRP"},{"code":"P366","type":"Legacy Concept Name","value":"Quantitative_Reverse_Transcriptase_PCR"},{"code":"P106","type":"Semantic_Type","value":"Molecular Biology Research Technique"},{"code":"P207","type":"UMLS_CUI","value":"C1514628"}]}