{"code":"C19641","name":"Dideoxy Chain Termination DNA Sequencing","terminology":"ncit","version":"26.09d","conceptStatus":"DEFAULT","leaf":true,"active":true,"synonyms":[{"name":"SANGER SEQUENCING","termType":"PT","type":"FULL_SYN","typeCode":"P90","source":"CDISC"},{"name":"Dideoxy Chain Termination DNA Sequencing","termType":"PT","type":"FULL_SYN","typeCode":"P90","source":"NCI"},{"name":"Sanger Sequencing","termType":"SY","type":"FULL_SYN","typeCode":"P90","source":"NCI"},{"name":"Dideoxy Chain Termination DNA Sequencing","type":"Preferred_Name","typeCode":"P108"}],"definitions":[{"definition":"A DNA sequencing technique in which a mixture of deoxynucleosidetriphosphates (dNTPs) and chain-terminating dNTPs (which are radioactively or fluorescently labeled) are combined within the reaction mixture. Once the polymerase chain reaction is complete the DNA strands are separated by size and the labeled chain terminating dNTPs can be read in sequence by the investigator or a machine.","code":"P325","type":"ALT_DEFINITION","source":"CDISC"},{"definition":"A DNA sequencing technique in which a mixture of deoxynucleosidetriphosphates (dNTPs) and chain-terminating dNTPs, which are radioactively or fluorescently labeled, are combined within the reaction mixture. Once the reaction is complete, the DNA strands are separated by size, and the labeled chain terminating dNTPs can be read in sequence by the investigator or by a machine.","code":"P97","type":"DEFINITION","source":"NCI"}],"properties":[{"code":"P322","type":"Contributing_Source","value":"CDISC"},{"code":"P366","type":"Legacy Concept Name","value":"Dideoxy_Chain_Termination_DNA_Sequencing"},{"code":"P106","type":"Semantic_Type","value":"Molecular Biology Research Technique"},{"code":"P207","type":"UMLS_CUI","value":"C1511897"}]}