{"code":"C192633","name":"Anchored Multiplex Polymerase Chain Reaction","terminology":"ncit","version":"26.09d","conceptStatus":"DEFAULT","leaf":true,"active":true,"synonyms":[{"name":"AMP","termType":"SY","type":"FULL_SYN","typeCode":"P90","source":"NCI"},{"name":"Anchored Multiplex PCR","termType":"SY","type":"FULL_SYN","typeCode":"P90","source":"NCI"},{"name":"Anchored Multiplex Polymerase Chain Reaction","termType":"PT","type":"FULL_SYN","typeCode":"P90","source":"NCI"},{"name":"Anchored Multiplex Polymerase Chain Reaction","type":"Preferred_Name","typeCode":"P108"}],"definitions":[{"definition":"A technique designed to rapidly enrich and amplify targets derived from samples that yield low levels of nucleic acids, such as formalin-fixed paraffin-embedded (FFPE) tissues, for use in next-generation sequencing. Briefly, total nucleic acid or genomic DNA (gDNA) is isolated from fresh or FFPE tissues. Total nucleic acid is converted to double-stranded cDNA (dscDNA) and cleaned by solid-phase reversible immobilization (SPRI). Then the dscDNA or gDNA is subjected to end repair, adenylation and ligation with a universal half-functional adapter. The modified DNA sample is then subjected to two rounds of nested low-cycle PCR, where the first round uses target gene-specific primers and the second round uses nested target gene-specific primers that are 5' tagged with a common sequencing adapter. The resulting target amplicon library is cleaned using SPRI and is suitable for use in next generation genomic assays.","code":"P97","type":"DEFINITION","source":"NCI"}],"properties":[{"code":"P208","type":"NCI_META_CUI","value":"CL1912943"},{"code":"P171","type":"PubMedID_Primary_Reference","value":"25384085"},{"code":"P106","type":"Semantic_Type","value":"Laboratory Procedure"}]}