{"code":"C159551","name":"Two-Stage Clotting Factor Assay","terminology":"ncit","version":"26.09d","conceptStatus":"DEFAULT","leaf":true,"active":true,"synonyms":[{"name":"Two-Stage Clotting Assay","termType":"SY","type":"FULL_SYN","typeCode":"P90","source":"NCI"},{"name":"Two-Stage Clotting Factor Assay","termType":"PT","type":"FULL_SYN","typeCode":"P90","source":"NCI"},{"name":"Two-Stage Clotting Factor Assay","type":"Preferred_Name","typeCode":"P108"}],"definitions":[{"definition":"A two-staged test with purified coagulation factors in the first stage, and a FXa-specific chromogenic substrate in the second stage. In the first stage, diluted sample plasma, which has been treated with Al(OH)3 to remove prothrombin, is mixed with a combined reagent (human serum, factor V, phospholipids and calcium). In the second stage, a subsample from the first stage is mixed with normal plasma, providing prothrombin and fibrinogen, and the clotting time is measured to deduce the amount of FVIII present.","code":"P97","type":"DEFINITION","source":"NCI"}],"properties":[{"code":"P208","type":"NCI_META_CUI","value":"CL951389"},{"code":"P106","type":"Semantic_Type","value":"Laboratory Procedure"}]}